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Detailed characterization of macrocyclic glycopeptide-based chiral stationary phases in SFC
Folprechtová, Denisa ; Kalíková, Květa (advisor) ; Křížek, Tomáš (referee) ; Kohout, Michal (referee)
The choice of a suitable chiral stationary phase is a key factor for successful enantioseparation in chromatographic methods. Macrocyclic glycopeptides are chiral selectors that, due to their unique structure, allow to interact with the analyte by various types of interactions simultaneously, which contributes to their complex retention and enantiodiscrimination mechanisms. Macrocyclic glycopeptides are widely used chiral stationary phases in liquid chromatography, but they have not been thoroughly tested in sub/supercritical fluid chromatography, which currently rank among important separation techniques in terms of chiral analyses. Therefore, the aim of this thesis is a detailed characterization of the enantiodiscrimination and retention mechanisms of macrocyclic glycopeptide-based chiral stationary phases packed with core-shell particles, namely TeicoShell, VancoShell, and NicoShell columns in sub/supercritical fluid chromatography. First, the effect of the mobile phase composition, especially the type and amount of organic modifiers and additives, on individual chromatographic parameters, i.e., retention, resolution of enantiomers, and peak shapes was studied in detail using selected sets of structurally different biologically active chiral compounds and materials. Based on the obtained...
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